Myelination on Mimetix scaffolds

Esmonde-White, C. et al. Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature OligodendrocytesJournal of Neuropathology & Experimental Neurology 78, 468–479 (2019).

M Swire, Y Korelevtsev, DJ Webb, DA Lyons, C Ffrench-Constant “Endothelin signalling mediates experience-dependent myelination in the CNS” eLife 2019

ME Bechler, “A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation” Oligodendrocytes (pp. 97-110). Humana Press, New York, NY. (2019)

D Allimuthu, Z Hubler, FJ Najm, H Tang, I Bederman, W Seibel,PJ Tesar, DJ Adams. (2019). Diverse Chemical Scaffolds Enhance Oligodendrocyte Formation by Inhibiting CYP51, TM7SF2, or EBP. Cell chemical biology, 26(4), 593-599.

A Dillenburg, G Ireland, RK Holloway, CL Davies, FL Evans, M Swire, ME Bechler, D Soong, T Yuen, G Su, J Bechler, C Smith, A Williams, VE Miron, Activin receptors regulate the oligodendrocyte lineage in health and disease“. Acta neuropathologica, 135(6), 887-906

MM Fu, TS McAlear, H Nguyen, JA Oses-Prieto, A Valenzuela, RD Shi, … S Bechstedt & BA Barres. (2019). The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for MyelinationCell (2019) 179 (1): 132-146.e14

Y Xu, D Chitsaz, RA Brown, QL Cui, MA Dabarno, JP Antel, T Kennedy, Deep learning for high-throughput quantification of oligodendrocyte ensheathment at single-cell resolution“.Communications Biology 2, Article number: 116 (2019)

AR Malheiro, B Correia, T Ferreira da Silva, D Bessa‐Neto, PP Van Veldhoven, P Brites. Leukodystrophy caused by plasmalogen deficiency rescued by glyceryl 1‐myristyl ether treatment. Brain Pathology (2019)

Ulc, A. et al. The guanine nucleotide exchange factor Vav3 modulates oligodendrocyte precursor differentiation and supports remyelination in white matter lesionsGlia 67, 376–392 (2018).

Harboe, M., Torvund-Jensen, J., Kjaer-Sorensen, K. & Laursen, L. S. Ephrin-A1-EphA4 signaling negatively regulates myelination in the central nervous systemGlia 66, 934–950 (2018).

MS Elitt et al. “Chemical Screening Identifies Enhancers of Mutant Oligodendrocyte Survival and Unmasks a Distinct Pathological Phase in Pelizaeus-Merzbacher Disease.” Stem cell reports 11.3 (2018): 711-726.

Z Hubler, D Allimuthu, I Bederman, MS Elitt, M Mudhavan, KC Allan, HE Shick, E Garrison, M Karl, DC Factor, ZS Nevin, JL Sax, MA Thompson, Y Fedorov, J Jin, WK Wilson, M Giera, F Bracher, RH Miller, PJ Tesar, DJ Adams, Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination “.Nature (2018), 560(7718),372

L Li, E Tian, X Chen, J Chao, J Klein, Q Qu, & Y Shi. (2018). GFAP mutations in astrocytes impair oligodendrocyte progenitor proliferation and myelination in an hiPSC model of Alexander disease. Cell stem cell, 23(2), 239-251.

Jadasz, J. J. et al. Human mesenchymal factors induce rat hippocampal- and human neural stem cell dependent oligodendrogenesisGlia 66, 145–160 (2017).

M Bechler, L Byrne, C Ffrench-Constant, “CNS Myelin Sheaths are an intrinsic property of Oligodendrocytes”. Current Biology 25, 1-6 September 21, 2015

Biomaterials and Tissue Engineering

Somayajulu, M. et al. Airborne Particulates Affect Corneal Homeostasis and Immunity. Investigative Opthalmology & Visual Science 61, 23 (2020).

Sawadkar P. et al. A Synergistic Relationship between Polycaprolactone and Natural Polymers Enhances the Physical Properties and Biological Activity of Scaffolds. ACS Applied Materials & Interfaces 12, 13587-13597 (2020)

N Kahli, P Sawadkar, S Ho, V Sharma, M Snow, S Powell, MA Woodruff, L Hook, E Garcia-Gareta “Pre-screening the intrinsic angiogenic capacity of biomaterials in an optimised ex ovo chorioallantoic membrane modelJournal of Tissue Engineering 2020

A Synergistic Relationship between Polycaprolactone and Natural Polymers Enhances the Physical Properties and Biological Activity of Scaffolds. ACS Applied Materials & Interfaces Available at: (Accessed: 18th May 2020)

A Bhartiya, K Madi, CM Disney, L Courtois, A Jupe, F Zhang, AJ Bodey, P Lee, C Rau, IK Robinson and M Yusuf “Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-raysJournal of Synchrotron Radiation 2019

Fernandez, T; Strudwick, XL; Al-Bataineh, S; Short, RD and Cowin, AJ. Surfaces to enhance matrix deposition for wound healing [online]. Wound Practice & Research: Journal of the Australian Wound Management Association, Vol. 26, No. 4, Dec 2018: 201-209.

C Ramachandran, V Sangwan, I Ortega, U Bhatnagar, S Mulla, R McKean, S MacNeil, Synthetic biodegradable alternatives to the use of the amniotic membrane for corneal regeneration: assessment of local and systemic toxicity in rabbits“. British journal of Ophthalmology 2018

R Santoro, S Venkateswaran, F AmadeoR Zhang, M Brioschi, A Callanan, M Agrifoglio, C Banfi, M Bradley, M Pesce, “Acrylate-based materials for heart valve scaffold engineering“. Biomater. Sci., 2018, 6, 154-167

HRB Raghavendran, S Puvaneswary, S Talebian, MR Murali, SV Naveen, G Krishnamurithy, R McKean, T Kamarul “A comparative study on in vitro osteogenic priming potential of electronspun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering applications” PLoS One 9, 8 (2014), e104389.

A. L. G. Millas, R. McKean, R. Stevens, M. Yusuf, J. V.W. Silveira, M. B. Puzzi, E. Bittencourt “Fabrication of electrospun scaffolds incorporating an Amazonian therapeutic oil from the Copaifera sp. for wound care applications” J. Biomater. Tissue Eng. 4 (2014), pp. 217-220.

Ortega, Í. et al. Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal RegenerationJournal of Visualized Experiments (2014). doi:10.3791/51826

Sefat, F. et al. An “off-the shelf” synthetic membrane to simplify regeneration of damaged corneas. 2nd Middle East Conference on Biomedical Engineering (2014). doi:10.1109/mecbme.2014.6783206

Liu, Y., Buckley, C. T., Almeida, H. V., Mulhall, K. J. & Kelly, D. J. Infrapatellar Fat Pad-Derived Stem Cells Maintain Their Chondrogenic Capacity in Disease and Can be Used to Engineer Cartilaginous Grafts of Clinically Relevant DimensionsTissue Engineering Part A 20, 3050–3062 (2014).

Sefat, F. et al. Production, Sterilisation and Storage of Biodegradable Electrospun PLGA Membranes for Delivery of Limbal Stem Cells to the CorneaProcedia Engineering 59, 101–116 (2013).

P. Deshpande, C. Ramachandran, F. Sefat, I. Mariappan, C. Johnson, R. McKean, M. Hannah, V. S. Sangwan, F. Claeyssens, A. J. Ryand, S. MacNeil “Simplifying corneal surface regeneration using a biodegradable synthetic membrane and limbal tissue explants” Biomaterials 34, 21 (2013), pp. 5088–5106.

Cell Culture

Cei, D. et al. Development of a dynamic in vitro stretch model of the alveolar interface with aerosol delivery. Biotechnology and Bioengineering (2020). doi:10.1002/bit.27600

Veronique Chotteau, Mattias Leino, Carolina Åstrand, Brendan Robb, and Robert McKean, “Impact of the dynamic culture system for 3D high cell density neural differentiation of hESC in electrospun PCL scaffolds” in “Advancing Manufacture of Cell and Gene Therapies VI”, Dolores Baksh, GE Healthcare, USA Rod Rietze, Novartis, USA Ivan Wall, Aston University, United Kingdom Eds, ECI Symposium Series, (2019)

  1. M.Leino, C. Astrand, N. Hughes-Brittain, B. Robb, R.McKean, V.Chotteau, Human embryonic stem cell dispersion in electrospun PCL fiber scaffolds by coating with laminin-521 and E-cadherin-Fc“.J.Biomed Mater Res Part B 2017

R. McKean, E.Heister, “Technology Platforms for 3D Cell Culture: A User’s Guide. Chapter 12“. Wiley. ISBN: 9781118851500

L. Al-Khalili,K. Gillner, Y. Zhang, C. Astrand,N. Hughes-Brittain, R.McKean, B. Robb, V.Chotteau, “Characterization of Human CD133+ cells in biocompatible poly(l-lactic acid) electrospun nano-fiber scaffolds“. Journal of Biomaterials and Tissue Engineering, Vol. 6, no 12, 959-966, 2016

A.J.Massey, “Tumour growth environment modulates Chk1 signalling pathways and Chk1 inhibitor sensitivity“. Nature Scientific Reports 2016

Brennan, M. Á. et al. 3D cell culture and osteogenic differentiation of human bone marrow stromal cells plated onto jet-sprayed or electrospun micro-fiber scaffoldsBiomedical Materials 10, 045019 (2015).

Bartok, M., Gabel, D., Zorn-Kruppa, M. & Engelke, M. Development of an in vitro ocular test system for the prediction of all three GHS categoriesToxicology in Vitro 29, 72–80 (2015).

Scaffold Characterisation

L Garcia, S Soliman, MP Francis, et al “Workshop on the characterisation of fiber-based scaffolds: challenges, progress and future directions“. J Biomed Mater Res. 2019; 1-10.

R.S.Bradley, I. K. Robinson, Y. Mohammed, “3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds“. Macromolecular Bioscience 2016

H. Suhaimi, S. Wang, T. Thornton, D. B. Das “On glucose diffusivity of tissue engineering membranes and scaffolds” Chemical Engineering Science 126 (2015), pp. 244–256.

M. Yusuf, A. L. G. Millas, A. K. C. Estandarte, G. K. Bhella, R. McKean, E. Bittencourt, I. K. Robinson “Platinum blue staining of cells grown in electrospun scaffolds” BioTechniques 57 (2014), pp.137-141.

I. Ortega, R. McKean, A. Ryan, S. MacNeil, F. Claeyssens “Characterisation and evaluation of the impact of microfabricated pockets on the performance of limbal epithelial stem cells in biodegradeable PLGA membranes for corneal regeneration” Biomater. Sci. 2 (2014), pp. 723-734.