Myelination on Mimetix scaffolds

Luo, J. X. X., Cui, Q.,Yaqubi M.,Hall, J.A., Dudley, R., Srour, M., Addour, N., Jamann, H., Larochelle, C., Blain, M., Healy, L. M., Stratton J. A., Sonnen, J. A., Kennedy T. E., Antel, J.P. Human Oligodendrocyte Myelination Potential; Relation to Age and DifferentiationAnnals of Neurology. 91(2), 178-191 (2021)

Swire, M., Assinck, P., McNaughton, P. A., Lyons, D. A., ffrench-Constant, C. & Livesey, M. R. Oligodendrocyte HCN2 Channels Regulate Myelin Sheath LengthJournal of Neuroscience 41 (38), 7954-7964 (2021).

Esmonde-White, C., Yaqubi, M., Bilodeau, P., Cui, Q., Pernin, F., Larochelle, C., Ghadiri, M., Xu, Y., Kennedy, T., Hall, J., Healy, L. & Antel, J. Distinct Function-Related Molecular Profile of Adult Human A2B5-Positive Pre-Oligodendrocytes Versus Mature Oligodendrocytes. Journal of Neuropathology & Experimental Neurology 78, 468-479 (2019).

Swire, M., Kotelevtsev, Y., Webb, D., Lyons, D. & ffrench-Constant, C. Endothelin signalling mediates experience-dependent myelination in the CNS. eLife 8, (2019).

Bechler, M. A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation.Oligodendrocytes 97-110 (2019). doi:10.1007/978-1-4939-9072-6_6

Allimuthu, D., Hubler, Z., Najm, F., Tang, H., Bederman, I., Seibel, W., Tesar, P. & Adams, D. Diverse Chemical Scaffolds Enhance Oligodendrocyte Formation by Inhibiting CYP51, TM7SF2, or EBP. Cell Chemical Biology 26, 593-599.e4 (2019).

Fu, M., McAlear, T., Nguyen, H., Oses-Prieto, J., Valenzuela, A., Shi, R., Perrino, J., Huang, T., Burlingame, A., Bechstedt, S. & Barres, B. The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination. Cell 179, 132-146.e14 (2019).

Xu, Y., Chitsaz, D., Brown, R., Cui, Q., Dabarno, M., Antel, J. & Kennedy, T. Deep learning for high-throughput quantification of oligodendrocyte ensheathment at single-cell resolution. Communications Biology 2, (2019). Read More

Malheiro, A., Correia, B., Ferreira da Silva, T., Bessa‐Neto, D., Van Veldhoven, P. & Brites, P. Leukodystrophy caused by plasmalogen deficiency rescued by glyceryl 1‐myristyl ether treatment. Brain Pathology 29, 622-639 (2019).

Dillenburg, A., Ireland, G., Holloway, R., Davies, C., Evans, F., Swire, M., Bechler, M., Soong, D., Yuen, T., Su, G., Becher, J., Smith, C., Williams, A. & Miron, V. Activin receptors regulate the oligodendrocyte lineage in health and diseaseActa Neuropathologica 135, 887-906 (2018).

Ulc, A., Zeug, A., Bauch, J., van Leeuwen, S., Kuhlmann, T., ffrench-Constant, C., Ponimaskin, E. & Faissner, A. The guanine nucleotide exchange factor Vav3 modulates oligodendrocyte precursor differentiation and supports remyelination in white matter lesionsGlia 67, 376–392 (2018).

Harboe, M., Torvund-Jensen, J., Kjaer-Sorensen, K. & Laursen, L. S. Ephrin-A1-EphA4 signaling negatively regulates myelination in the central nervous systemGlia 66, 934–950 (2018).

Elitt, M., Shick, H., Madhavan, M., Allan, K., Clayton, B., Weng, C., Miller, T., Factor, D., Barbar, L., Nawash, B., Nevin, Z., Lager, A., Li, Y., Jin, F., Adams, D. & Tesar, P. Chemical Screening Identifies Enhancers of Mutant Oligodendrocyte Survival and Unmasks a Distinct Pathological Phase in Pelizaeus-Merzbacher Disease. Stem Cell Reports 11, 711-726 (2018).

Hubler, Z., Allimuthu, D., Bederman, I., Elitt, M., Madhavan, M., Allan, K., Shick, H., Garrison, E., T. Karl, M., Factor, D., Nevin, Z., Sax, J., Thompson, M., Fedorov, Y., Jin, J., Wilson, W., Giera, M., Bracher, F., Miller, R., Tesar, P. & Adams, D. Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination. Nature 560, 372-376 (2018). Read More

Li, L., Tian, E., Chen, X., Chao, J., Klein, J., Qu, Q., Sun, G., Sun, G., Huang, Y., Warden, C., Ye, P., Feng, L., Li, X., Cui, Q., Sultan, A., Douvaras, P., Fossati, V., Sanjana, N., Riggs, A. & Shi, Y. GFAP Mutations in Astrocytes Impair Oligodendrocyte Progenitor Proliferation and Myelination in an hiPSC Model of Alexander Disease. Cell Stem Cell 23, 239-251.e6 (2018).

Jadasz, J., Tepe, L., Beyer, F., Samper Agrelo, I., Akkermann, R., Spitzhorn, L., Silva, M., Oreffo, R., Hartung, H., Prigione, A., Rivera, F., Adjaye, J. & Küry, P.  Human mesenchymal factors induce rat hippocampal- and human neural stem cell dependent oligodendrogenesisGlia 66, 145–160 (2017).

Bechler, M., Byrne, L. & ffrench-Constant, C. CNS Myelin Sheath Lengths Are an Intrinsic Property of Oligodendrocytes. Current Biology 25, 2411-2416 (2015). Read More


Biomaterials and Tissue Engineering

Ramachandran, C., Deshpande, P., Ortega, I., Farshid, S., Mckean, R., Srivastava, M., MacNeil, S., Basu, S., Sangwan, V. S. Proof-of-concept study of electrospun PLGA membrane in the treatment of limbal stem cell deficiency. BMJ Open Ophthalmology  6, 1 (2021) doi: 10.1136/bmjophth-2021-000762.

Somayajulu, M., Ekanayaka, S., McClellan, S., Bessert, D., Pitchaikannu, A., Zhang, K. & Hazlett, L. Airborne Particulates Affect Corneal Homeostasis and Immunity. Investigative Opthalmology & Visual Science 61, 23 (2020).

Sawadkar, P., Mohanakrishnan, J., Rajasekar, P., Rahmani, B., Kohli, N., Bozec, L. & García-Gareta, E. A Synergistic Relationship between Polycaprolactone and Natural Polymers Enhances the Physical Properties and Biological Activity of Scaffolds. ACS Applied Materials & Interfaces 12, 13587-13597 (2020). Read More

Kohli, N., Sawadkar, P., Ho, S., Sharma, V., Snow, M., Powell, S., Woodruff, M., Hook, L. & García-Gareta, E. Pre-screening the intrinsic angiogenic capacity of biomaterials in an optimised ex ovo chorioallantoic membrane model. Journal of Tissue Engineering 11, 204173142090162 (2020). Read More

Bhartiya, A., Madi, K., Disney, C., Courtois, L., Jupe, A., Zhang, F., Bodey, A., Lee, P., Rau, C., Robinson, I. & Yusuf, M. Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-raysJournal of Synchrotron Radiation 27, 158-163 (2020). Read More

Keller, L., Pijnenburg, L., Idoux-Gillet, Y., Bornert, F., Benameur, L., Tabrizian, M., Auvray, P., Rosset, P., María Gonzalo-Daganzo, R., Gómez Barrena, E., Gentile, L. & Benkirane-Jessel, N. Preclinical safety study of a combined therapeutic bone wound dressing for osteoarticular regeneration. Nat Commun 10, 2156 (2019).

Fernandez, T., Strudwick, XL., Al-Bataineh, S., Short, R.D., and Cowin, A.J. Surfaces to enhance matrix deposition for wound healing. Wound Practice & Research: Journal of the Australian Wound Management Association 26, 201-209 (2018).

Ramachandran, C., Sangwan, V., Ortega, I., Bhatnagar, U., Mulla, S., McKean, R. & MacNeil, S. Synthetic biodegradable alternatives to the use of the amniotic membrane for corneal regeneration: assessment of local and systemic toxicity in rabbitsBritish Journal of Ophthalmology 103, 286-292 (2018). Read More

Santoro, R., Venkateswaran, S., Amadeo, F., Zhang, R., Brioschi, M., Callanan, A., Agrifoglio, M., Banfi, C., Bradley, M. & Pesce, M. Acrylate-based materials for heart valve scaffold engineering. Biomaterials Science 6, 154-167 (2018).

Balaji Raghavendran, H., Puvaneswary, S., Talebian, S., Raman Murali, M., Vasudevaraj Naveen, S., Krishnamurithy, G., McKean, R. & Kamarul, T. A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application. PLoS ONE 9, e104389 (2014). Read More

Millas, A., McKean, R., Stevens, R., Yusuf, M., Silveira, J., Puzzi, M. & Bittencourt, E. Fabrication of Electrospun Scaffolds Incorporating an Amazonian Therapeutic Oil from the Copaifera Species for Wound Care Applications. Journal of Biomaterials and Tissue Engineering 4, 217-220 (2014). Read More

Ortega, Í., Sefat, F., Deshpande, P., Paterson, T., Ramachandran, C., Ryan, A., MacNeil, S. & Claeyssens, F. Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal RegenerationJournal of Visualized Experiments (2014). doi:10.3791/51826

Sefat, F., Ortega, I., McKean, R., Deshpande, P., Ramachandran, C., Hill, C., Tzokov, S., Claeyssens, F., Sangwan, V., Ryan, A. & MacNeil, S. An “off-the shelf” synthetic membrane to simplify regeneration of damaged corneas. 2nd Middle East Conference on Biomedical Engineering (2014). doi:10.1109/mecbme.2014.6783206

Liu, Y., Buckley, C. T., Almeida, H. V., Mulhall, K. J. & Kelly, D. J. Infrapatellar Fat Pad-Derived Stem Cells Maintain Their Chondrogenic Capacity in Disease and Can be Used to Engineer Cartilaginous Grafts of Clinically Relevant DimensionsTissue Engineering Part A 20, 3050–3062 (2014).

Sefat, F., McKean, R., Deshpande, P., Ramachandran, C., Hill, C., Sangwan, V., Ryan, A. & MacNeil, S. Production, Sterilisation and Storage of Biodegradable Electrospun PLGA Membranes for Delivery of Limbal Stem Cells to the CorneaProcedia Engineering 59, 101–116 (2013).

Deshpande, P., Ramachandran, C., Sefat, F., Mariappan, I., Johnson, C., McKean, R., Hannah, M., Sangwan, V., Claeyssens, F., Ryan, A. & MacNeil, S. Simplifying corneal surface regeneration using a biodegradable synthetic membrane and limbal tissue explants. Biomaterials 34, 5088-5106 (2013). Read More


Cell Culture

Cei, D., Doryab, A., Lenz, A., Schröppel, A., Mayer, P., Burgstaller, G., Nossa, R., Ahluwalia, A. & Schmid, O. Development of a dynamic in vitro stretch model of the alveolar interface with aerosol delivery. Biotechnology and Bioengineering (2020). doi:10.1002/bit.27600

Veronique Chotteau, Mattias Leino, Carolina Åstrand, Brendan Robb, and Robert McKean,Impact of the dynamic culture system for 3D high cell density neural differentiation of hESC in electrospun PCL scaffolds” in “Advancing Manufacture of Cell and Gene Therapies VI”, Dolores Baksh, GE Healthcare, USA Rod Rietze, Novartis, USA Ivan Wall, Aston University, United Kingdom Eds, ECI Symposium Series, (2019)

Aladdad, A., Amer, M., Sidney, L., Hopkinson, A., White, L., Alexander, C. & Rose, F. A thermoresponsive three-dimensional fibrous cell culture platform for enzyme-free expansion of mammalian cells. Acta Biomaterialia 95, 427-438 (2019). Read More

  1. Leino, M., Astrand, C., Hughes‐Brittain, N., Robb, B., McKean, R. & Chotteau, V. Human embryonic stem cell dispersion in electrospun PCL fiber scaffolds by coating with laminin‐521 and E ‐cadherin‐ F c. Journal of Biomedical Materials Research Part B: Applied Biomaterials 106, 1226-1236 (2017). Read More

McKean, R. &, Heister, E. Technology Platforms for 3D Cell Culture: A User’s Guide. (Wiley, 2017) ISBN: 9781118851500

Al-Khalili, L., Gillner, K., Zhang, Y., Åstrand, C., Shokri, A., Hughes-Brittain, N., McKean, R., Robb, B. & Chotteau, V. Characterization of Human CD133+ Cells in Biocompatible Poly(l-lactic acid) Electrospun Nano-Fiber Scaffolds. Journal of Biomaterials and Tissue Engineering 6, 959-966 (2016). Read More

Wayne, J., Brooks, T. & Massey, A. Inhibition of Chk1 with the small molecule inhibitor V158411 induces DNA damage and cell death in an unperturbed S-phaseOncotarget 7, 85033-85048 (2016). Read More

Massey, A. Tumour growth environment modulates Chk1 signalling pathways and Chk1 inhibitor sensitivity. Science Report 6, 35874 (2016)

Brennan, M. Á. et al. 3D cell culture and osteogenic differentiation of human bone marrow stromal cells plated onto jet-sprayed or electrospun micro-fiber scaffoldsBiomedical Materials 10, 045019 (2015).

Bartok, M., Gabel, D., Zorn-Kruppa, M. & Engelke, M. Development of an in vitro ocular test system for the prediction of all three GHS categoriesToxicology in Vitro 29, 72–80 (2015).


Scaffold Characterisation

Garcia, L., Soliman, S., Francis, M., Yaszemski, M., Doshi, J., Simon, C. & Robinson‐Zeigler, R. Workshop on the characterization of fiber‐based scaffolds: Challenges, progress, and future directions. Journal of Biomedical Materials Research Part B: Applied Biomaterials 108, 2063-2072 (2019).

Bradley, R., Robinson, I. & Yusuf, M. 3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds. Macromolecular Bioscience 17, 1600236 (2016). Read More

Suhaimi, H., Wang, S., Thornton, T. & Das, D. On glucose diffusivity of tissue engineering membranes and scaffolds. Chemical Engineering Science 126, 244-256 (2015).

Yusuf, M., Millas, A., Estandarte, A., Bhella, G., McKean, R., Bittencourt, E. & Robinson, I. Platinum blue staining of cells grown in electrospun scaffolds. BioTechniques 57, (2014).

Ortega, I., McKean, R., Ryan, A., MacNeil, S. & Claeyssens, F. Characterisation and evaluation of the impact of microfabricated pockets on the performance of limbal epithelial stem cells in biodegradable PLGA membranes for corneal regenerationBiomaterials Sci.ence 2, 723-734 (2014).