3D X-Ray Nanotomography of cells grown in Mimetix scaffolds

A publication by Robert S. Bradley, Ian K. Robinson and Mohammed Yusuf in Macromolecular Bioscience demonstrates that X-ray nanotomography with Zernike phase contrast can be used for 3D imaging of cells grown on electrospun polymer scaffolds. The scaffold fibers and cells are simultaneously imaged, enabling the influence of scaffold architecture on cell location and morphology to be studied. The high resolution enables subcellular details to be revealed. The X-ray imaging conditions were optimized to reduce scan times, making it feasible to scan multiple regions of interest in relatively large samples. An image processing procedure is presented which enables scaffold characteristics and cell location to be quantified. The publication can be downloaded here

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