3D Imaging of Cancer Cells in Scaffolds

Mimetix® scaffolds were used in the recent publication “Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays” in The Journal of Synchrotron Radiation. This study, led by a team at the University College London (UCL), collaborating with the Research Centre at Harwell (RCAH) and Diamond Light Source on the Harwell Innovation Campus, investigated the use of synchrotron x-ray method to determine the growth of cells in 3D structures. Mimetix® scaffolds are made of electrospun poly l-lactide (PLLA) and are used for cell culture and tissue engineering applications. The team compared two fibre diameters, including 4 microns which is used in commercially available Mimetix products, and examined cell infiltration and cell-cell connectivity over 2, 5 and 8 days. This investigation shows the potential of Mimetix® plates to study complex behaviour of cells in 3D and the potential for imaging technology to study cell-scaffold interactions for future medical use.

You can read more publication on electrospinning method used for tissue engineering applications here.

Disclaimer: Image reproduced with permission of the International Union of Crystallography. Original article source: Bhartiya et al. (2020) ‘Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays’, Journal of Synchrotron Radiation. doi: 10.1107/S1600577519015583
  • Last updated: April 24, 2020
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