Mimetix (PLLA) electrospun sheets are cut into micro-scaffolds on to which cells can be seeded, grown and differentiated prior to performing more conventional assays in well plates. Cells grow on, around and into the material, forming micro-islands of adherent cells that are effectively “micro-tissues in solution”. Iron particles are incorporated into the fibres to create scaffolds that can be physically manipulated using magnetism.

  • Reproducible 3-D culture environment
  • Applicable to any cell type (recombinant, human primary cells and iPSC’s plus differentiation)
  • Movable: from vessels-to-well and well-to-well with magnetism
  • Scalable to any assay throughput
  • Integrates seamlessly with current screening and assay workflows
Mimetix sheet cut by laser into 250 micron x 50 micron microscaffolds
Microscaffold with A549 cells 4 days post-seeding and unseeded scaffold (SEM)
C3H/10T1/2 fibroblast cells inoculated on to microscaffolds and incubated for 24hrs. Rhodamine dye incorporated into fibres. Red fibres. Green nuclei. Cells on and in the scaffold. 20x mag.
HEK293 fibroblast cells inoculated on to microscaffolds and incubated for 24hrs. Red fibres, green cell nucleus, purple/blue tubulin. Light sheet microscopy. 20x mag.
HEK293 fibroblast cells inoculated on to microscaffolds and incubated for 24hrs. Light sheet microscopy.
Recombinant HEK293 cells stably expressing CRE-luciferase (Promega) – monitors cyclic AMP (cAMP) levels in cells. Treatment with forskolin increases cAMP levels, which acts as a transcription factor and increases synthesis of luciferase. Experiment performed by Aurelia Bioscience.
Scaffold containing cells were defrosted and incubated in the Biowiggler for 24, 48 and 72 hrs prior to assay – only 72 hr data shown but data was consistent between these time points. Experiment by Aurelia Bioscience.

We developed microscaffolds in a collaborative project with Aurelia Bioscience. Project was supported by an InnovateUK/ NC3Rs grant.

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