Mimetix® electrospun scaffolds

The Electrospinning Company manufactures a range of scaffolds that are available in standard well-plate formats. Mimetix scaffolds are manufactured from GMP-grade poly-L-lactide polymer (PLLA) with tight control over key parameters such as fibre diameter and thickness. The laboratory consumable products are highly consistent providing researchers reliable tools for reproducible cell-based assays. They are provided irradiated with gamma or e-beam.

For more details on the product range click here. To purchase Mimetix products contact our distributor AMS Biotechnology.

 

Randomly orientated Mimetix® scaffold

Mimics the extracellular matrix while providing a true 3D environment for the cells to grow. Evaluated with a range of immortalised cell lines, primary cells and stem cells. Applications include: non weight-bearing bone regeneration; corneal repair; neurosciences.

Mimetix standard scaffold dimensions:

  • Fibre diameter of 4 microns
  • Pore size of 15-30 microns, porosity of around 80%
  • Depth of 50 microns
  • Available formats:
    • 96-well plate fixed scaffold (TECL-002)
    • 12-well plate with loose scaffolds and retaining rings (TECL-003)
    • Sheets or discs (to order)
96-well plate containing random scaffold
Randomly aligned fibres
Composite scaffold of PLLA and courtesy of Dr Raghavendran, University of Malaya
384-well plate with aligned scaffold
Aligned fibres
Liver cancer cells (HepG2) grown for 21 days in Mimetix scaffold

Aligned Mimetix® scaffold

Ideal for cells needing physical guidance and/or where cellular orientation influences cell behaviour and function.Evaluated with a range of primary cells and stem cells. Applications include: Schwann cells and oligodendrocytes, myelination assays, nerve conduit repair, tendon repair, cardiomyocytes.

Mimetix aligned scaffold standard dimensions:

  • Fibre diameter of 2 microns
  • Depth of 2-4 microns
  • Available formats:
    • 96-well plate fixed scaffolds (TECL-005),
    • 384-well plate fixed scaffold including rhodamine in the fibres (TECL-015)
    • Crown cell inserts in 12 or 24 well plates (TECL006)
Schwann cells grown for 21 days, courtesy of Dr Bechler, Centre for Regenerative Medicine, Edinburgh
Cardiomyocytes grown for 12 days on Aligned Mimetix scaffolds, courtesy of Dr Trepakova, Merck, USA

Extra porous Mimetix® air scaffold

Ideal for flow and bioreactor applications. Evaluated for stem cell (iPS and hESC) proliferation and differentiation.

Mimetix Air standard dimensions:

  • Fibre diameter of 7-8 microns
  • Porosity of over 90%
  • Depth of up to 3.5 mm
  • Available as discs or in well plate formats
Highly porous Mimetix air scaffold
Cross section of 2.5 mm scaffold showing complete penetration with muscle satellite cells, courtesy of Dr Chotteau, KTH, Sweden

Mimetix products can be purchased from AMS biotechnology.