Differentiation of neural stem cells into mature neurons in the Mimetix scaffold

Differentiation of neural stem cells into mature neurons within the Mimetix scaffold. Cells are stained with DCX (red) Tubulin ╬▓III (green) DAPI (blue).

Neurite outgrowth on our Mimetix scaffold. Cells are stained with DAPI (blue), Nestin (green) and GFAP (red).

Images: Courtesy of ReNeuron.

hES cells were differentiated for 21 days, RNA expression was analysed with RT-qPCR.

Nanog (pluripotency marker) is no longer expressed after 21 days. On the other hand; nestin, bIII-tubulin (two early neuronal precursors) as well as GAD67 and MAP2 (two post mitotic markers) are expressed.

Work performed at KTH, Sweden

hESC grow well and remain fully pluripotent in the Mimetix scaffold

hES cells were grown for 72h00 in the Mimetix scaffold.

Cells are growing well and cell number increases over 5-fold in 72h00. Pluripotency is assessed with Nanog (green) or Oct3/4 (red).

Experimental work performed at KTH, Sweden

An homogenous early neural progenitor cell population is obtained in only 5 days when the scaffolds are placed in bioreactors.

Pax6, a neural progenitor marker, gene expression increases 400 fold in 5 days in the Mimetix scaffold.

Work performed at KTH, Sweden

The majority of cells are differentiated into neural progenitors at day 5 (pax6 staining in green). Only a few still express Oct4 (in red) a marker for undifferentiated eSC.

Work performed at KTH, Sweden

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