HIGH THROUGHPUT MYELINATION ASSAY
Our aligned electrospun Mimetix scaffold allows:
SEM image of the aligned 2 μm diameter PLLA fibres in the Mimetix scaffold
Cortical oligodendrocytes precursors differentiating into active oligodendrocytes and lay myelin on the Mimetix scaffold in the absence of neurons. Cells are stained after 14 days with myelin basic protein (green) and Hoechst (blue).
Percent of oligodendrocyte precursors (NG2) and oligodendrocytes (MBP). More than 600 cells were counted per condition. Mean and standard deviation are shown for three experiments.
Schwann cells were grown for 21 days and stained with anti S-100 (purple) and Hoechst (blue)