Primary dermal fibroblasts were cultured on our randomly orientated fibre scaffold and images were taken after 24 hours and 7 days. After 7 days in culture, cells had proliferated extensively to fill the pores within the scaffold.
Images of primary dermal fibroblast growth on our electrospun scaffolds at 24 hours and 7 days after seeding.
Primary dermal fibroblast migration was studied by taking a cross-section of the scaffold showing that cells had migrated through the loose fibrous network to form a 3D structure.
Cross-section of our randomly orientated electrospun fibres containing human primary fibroblasts which have proliferated and migrated to form a 3D cell structure. Cells are stained with DAPI to indicate the nuclei.
An in vitro model of skin was produced by culturing primary keratinocytes alongside primary fibroblasts, indicating that non-woven electrospun scaffolds are a suitable substrate for co-cultures of primary cells.
Co-culture of primary dermal fibroblasts and keratinoctyes on our electrospun scaffolds.
