3D tumour environment affects DNA damage

In “Inhibition of Chk1 with the small molecule inhibitor V158411 induces DNA damage and cell death in an unperturbed S-phase. Joanne Wayne, Teresa Brooks, Andrew J. Massey. DOI: 10.18632/oncotarget.13119″ Vernalis researchersfollowed the regulation of the Chk1 and Chk6 kinases in various human tumour cell lines grown in 2D conditions, spheroids and Mimetix scaffolds and compared those data to what is observed in xenogratf models. they demonstrated that the tumour growth environment does modulate Chk1 signalling pathways and Chk1 inhibitor sensitivity. Cancer cells grown in the Mimetix scaffold responded to DNA damage similarly to cells grown as spheroids but differently to a 2D situation.


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