3D tumour environment affects DNA damage

Cancer cells grown in 3D in Mimetix scaffolds or spheroid cultures responded to DNA damage  differently to those grown in conventional 2D culture.

In “Inhibition of Chk1 with the small molecule inhibitor V158411 induces DNA damage and cell death in an unperturbed S-phase. Joanne Wayne, Teresa Brooks, Andrew J. Massey. DOI: 10.18632/oncotarget.13119″ Vernalis researchers followed the regulation of the Chk1 and Chk6 kinases in various human tumour cell lines grown in 2D conditions, spheroids and Mimetix 3D scaffolds and compared those data to what is observed in xenograft models. They demonstrated that the tumour growth environment does modulate Chk1 signalling pathways and Chk1 inhibitor sensitivity.

 

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